The National Genomics Data Center (NGDC), as part of the China National Center for Bioinformation (CNCB), provides a suite of database resources for worldwide researchers. As multi-omics big data and artificial intelligence reshape the paradigm of biology research, CNCBNGDC continuously updates its database resources to enhance data usability, foster knowledge discovery, and support data-driven innovative research. Over the past year, notable progress has been achieved in expanding the scope of high-quality multi-omics datasets, building new database resources, and optimizing extant core resources. Notably, the launch of BIG Search enables cross-database search services for large-scale biological data platforms, including NGDC, National Center for Biotechnology Information (NCBI), and European Bioinformatics Institute (EBI). Additionally, several new resources have been developed, covering genome and variation (Hiland Resource, TOAnnoPriDB), expression (TEDD), single-cell omics (PreDigs, scMultiModalMap, TE-SCALE), radiomics (TonguExpert), health and disease (CAVDdb, IDP, MTB-KB, ResMicroDb), biodiversity and biosynthesis (SugarcaneOmics), as well as research tools (Dingent, miMatch, OmniExtract, RDBSB, xmarkerFinder). All these resources and services are freely accessible at https://ngdc.cncb.ac.cn. [GRAPHICS] .